Journal: Nucleic Acids Research

Article Title: RDML: structured language and reporting guidelines for real-time quantitative PCR data

doi: 10.1093/nar/gkp056

Figure Lengend Snippet: Real-time PCR data exchange with RDML. RDML acts as a facilitator of, and common language for exchange of qPCR data and related information between users, software programs and databases.

Article Snippet: The RDML project is supported by companies providing real-time PCR instruments and reagents (Bio-Rad Laboratories, Roche, Applied Biosystems) or reagents only (Eurogentec, Primer Design), developing PCR data analysis software (Biogazelle, LabonNet and MultiD Analysis) and organizing qPCR courses (Biogazelle, TATAA Biocenter), as well as a number of opinion leaders, core facility managers and researchers active in the field of qPCR, including the developers of geNorm, qBase, RTprimerDB and REST.

Techniques: Real-time Polymerase Chain Reaction, Software

Journal: Microbiology Spectrum

Article Title: Randomized, Double-Blind, Placebo-Controlled Trial of a Throat Spray with Selected Lactobacilli in COVID-19 Outpatients

doi: 10.1128/spectrum.01682-22

Figure Lengend Snippet: Consolidated Standards of Reporting Trials (CONSORT) flowchart for patient recruitment and enrollment. *, excluded for not meeting the inclusion criteria (mostly because the time from a positive PCR test was >96 h, often when people contacted the study coordinator in response to the press release message) or declining to participate after the first contact with the study coordinator. Of note, for the analysis of SARS-CoV-2 loads by RT-qPCR, 4 participants in the verum group and 3 participants in the placebo group already had a negative PCR test at T 1 of our study. Hence, for this analysis, 30 participants and 27 participants were analyzed for verum and placebo, respectively.

Article Snippet: Furthermore, specific monitoring of these applied lactobacilli strains showed that they were detectable via quantitative PCR (qPCR) analysis in 82% of the patients in the verum group.

Techniques: Quantitative RT-PCR

Journal: Microbiology Spectrum

Article Title: Randomized, Double-Blind, Placebo-Controlled Trial of a Throat Spray with Selected Lactobacilli in COVID-19 Outpatients

doi: 10.1128/spectrum.01682-22

Figure Lengend Snippet: Microbial community composition in the airways (A), detection of the administered Lactobacillaceae strains (B and C), and association of treatment and symptom scores with bacterial taxa (D). (A) PCoA was used to visualize the microbiome composition in combined nose-throat swabs for each treatment group and at different time points. T 1 , start of the trial; T 2 , after 1 week; T 3 , after 2 weeks; T 4 , end of the trial. (B) Relative abundances of L. casei ASV1, L. plantarum ASV2, and L. rhamnosus ASV3 between the placebo and verum groups. See also Table S2 in the supplemental material for the mean relative abundances for all ASVs at different time points and statistics. (C) qPCR with species-specific primers for L. rhamnosus GG, L. casei AMBR2, and L. plantarum WCFS1 was used to estimate the counts of CFU per milliliter. Based on the standard curve, the detection limit was estimated to be 10 3 CFU/mL. (D) Association of treatment and symptom scores with bacterial taxa across all time points in the study. For treatment, the association was also evaluated for treatment time only.

Article Snippet: Furthermore, specific monitoring of these applied lactobacilli strains showed that they were detectable via quantitative PCR (qPCR) analysis in 82% of the patients in the verum group.

Techniques: